Peptides having immunomodulatory activity

ABSTRACT

The present invention relates to peptides having an immunomodulatory activity. The present invention is concerned with the tetrapeptide H-Lys-Asn-Pro-Tyr-OH (SEQ.ID NO:1) and analogues thereof as immunomodulators. The present invention also includes the use of such peptides as vaccine adjuvant.

FIELD OF THE INVENTION

The present invention relates to peptides having an immunomodulatory activity. In particular, the present invention relates to peptides which stimulate the immune system in animals, including humans. The present invention is concerned with the tetrapeptide H-Lys-Asn-Pro-Tyr-OH (SEQ ID NO:1) and analogues thereof as immunomodulators.

The present invention also includes compositions containing such immunomodulatory peptides and the use of such peptides and compositions as immunomodulators.

The present invention also includes the use of such peptides as a vaccine adjuvant.

BACKGROUND OF THE INVENTION

The primary function of the immune system relates to the protection of the body from disease. The immune system protects against not only those diseases which result from an attack by bacteria, virus, and other pathogens, but also cancer, as well as disease states which result from immune imbalance; opportunistic infections, or autoimmune disorders.

In a number of diseases or other pathological conditions, the immune system response of an animal or human is depressed. As a result, the subject becomes more susceptible to malignancies, or pathological infections against which a normal immune system would have protected the subject. Some conditions which depress the immune system include acquired immune deficiency syndrome (AIDS), chronic infection, chemotherapy, trauma of surgery, and aging.

Modulation of the immune system through pharmaceutically induced stimulation or suppression offers an important approach to the control of disease. Compounds which nonspecifically stimulate the immune system are of potentially significant medicinal importance and have been the object of lengthy research efforts. Often, the research results show that immunomodulating compounds are either weak immunostimulants, and therefore not very effective, or potent immunostimulants and, therefore, effective but toxic by virtue of this potent immunostimulating activity.

Among the many classes of compounds which non-specifically stimulate the immune system are a number of naturally occurring peptides or fragments thereof. One such fragment is Imreg, a tripeptide of the general sequence Tyr-Gly-Gly disclosed in U.S. Pat. No. 4,699,898 issued Oct. 13, 1987. This patent does not disclose the sequence of the peptides of the present invention. Imreg can be isolated from human leukocytes. It is a thymomimetic but possesses weak potency. (A. Arthur Gottlieb, Int.J.Immunopharm.Vol.13, Suppl.1, p.29-32 (1991)).

Another fragment of a naturally occurring peptide is the tetrapeptide Tuftsin and its close analogue Rigin. These peptides have the respective sequences Thr-Lys-Pro-Arg and Gly-Gln-Pro-Arg. Analogues of tuftsin can be found, for example, in Biondi et al., "Synthesis of glycosylated tuftsins and tuftsin-containing IgG fragment undecapeptide", Int.J.Peptide Protein Res. 37, 1991, p.112-121, and Verdini et al., "Immunostimulation by a partially modified retro-inverso-Tuftsin analogue containing Thr¹ ψ[NHCO](R,S)Lys² modification", J.Med.Chem., 1991 34, p.3372-3379. These publications do not disclose peptides with the sequence of the present invention. Tuftsin and rigin are both fragments of IgG and are found within the heavy chain of leukokinin. Tuftsin is a well-known macrophage activator and is known to stimulate NK cell activity. However, it is unstable in plasma which may strongly reduce its stimulating effects on the immune system.

Another fragment of a naturally occurring peptide known to have immunomodulating effects is thymopentin. (Audhya et al., "Contrasting biological activities of thymopoietin and splenin, two closely related polypeptide products of thymus and spleen", Proc.Natl.Acad.Sci.USA, 81,p.2847-2849, 1984; U.S. Pat. No. 5,218,089 issued Jun. 8, 1993; U.S. Pat. No. 5,091,510 issued Feb. 25, 1992). This pentapeptide has the sequence Arg-Lys-Asp-Val-Tyr. Thymopoietin is a 49 amino acid polypeptide thymus hormone. The pentapeptide is the smallest fragment of thymopoietinq which retains the immunological activity of the parent hormone. Thymopentin is a thymomimetic of weak potency. It induces phenotypic differentiation of T cells and increases Interleukin-2 production. Splenin, an analogue of thymopentin, affects both B and T cells. Splenin has the sequence Arg-Lys-Glu-Val-Tyr in bovine and Arg-Lys-Ala-Val-Tyr in humans. Another similar peptide is thymotrinan which has the sequence Arg-Lys-Asp. While these peptides also produce immunomodulating effects, they do not contain or disclose the sequence of the peptides of the present invention.

Another naturally occurring compound is Eisenin extracted from a brown marine algae. This tripeptide has the sequence pyroGlu-Gln-Ala. It was reported in Kojima et al., "Eisenin (L-pyroGlu-L-Gln-L-Ala), a new biological response modifier", Journal of Immunotherapy, 13, p.36-42, 1993 that Eisenin showed immunological activity augmenting natural cytotoxicity of peripheral blood lymphocytes (PBLs) in humans. The natural cytotoxicity augmented by Eisenin seems to be due mainly to NK cells.

One peptide named "LANT 6" has the sequence Lys-Asn-Pro-Tyr-Ile-Leu. LANT6 has been found in ganglion cells and is believed to play a role in neurotransmission between retina ganglion cells and their central target areas. LANT 6 possesses some sequence homology with neurotensin. Neurotensin analogues have been described as cerebral medicaments, antipsychotic agents, analgesics, and anphetamine antagonists. However, LANT 6 has not been reported to have immunomodulatory activity.

Furthermore, it is known in the peptide art, as noted in U.S. Pat. No. 4,426,324 "Immunopotentiating Peptides" issued Jan. 17, 1984, that deletion of even one amino acid from the sequence of a biologically active peptide can result in the loss of biological activity. Therefore, the inclusion of the sequence of the present invention in the sequence of LANT 6 for use as a neuropeptide does not indicate that a shorter sequence within LANT6 would be active as an immunomodulator.

It is well known that vaccines are important in the prophylaxis of disease. Vaccines operate by exposing a host animal to foreign material designed to activate the immune system to confer upon the host immunity against the material without exposing the host to the risk of disease. At the present time, about 20 vaccines have been developed for commercial use. Some of these vaccines are made by detoxification of the toxin produced by the microorganism, or a portion of that organism, or by isolation of a specific non-toxic portion of the organism. A well known example of the latter is the isolation of capsular polysaccharides from meningococcal and pneumococcal bacteria as the basis for a vaccine for bacterial meningitis and pneumonia. However, polysaccharide vaccines are poor immunogens which do not give rise to adequate amounts of protective antibodies in individuals with poorly developed or impaired immune systems. The latter includes young children, the elderly, or those with autoimmune diseases. Furthermore, the immune response which does occur is T-independent or non-memory which means the individual will not display an increased antibody response, with seroconversion, upon being given a booster injection. T-cell dependence is necessary for the induction of IgG antibodies and memory cells. Thus, upon seroconversion, both IgM and IgG antibodies are formed upon repeated injections of a vaccine. Furthermore, the magnitude of the antibody response increases with each injection of vaccine, when the response is T-dependent. The immunology of polysaccharide vaccines has been reviewed by Kennings et al, "The Polysaccharides" (Editor; GO Spinal), Volume 1, 291-329 (1982).

As regards adjuvants for commercial use, only aluminum and calcium salts are presently employed as adjuvants. However, aluminum and calcium salts are not potent adjuvants. Calcium salts have found only limited use. While aluminum salts have found more widespread use with other vaccines, little success has been reported with polysaccharide protein conjugate vaccines. In fact, it has been reported that aluminum hydroxide inhibits the antibody response to an H. Influenzae b polysaccharide-tetanus toxoid conjugate vaccine; J. B. Robbins, et al also observe the same suppression of the antibody response with aluminum hydroxide and a S Typhi polysaccharide--cholera toxin conjugate vaccine; J. Experimental Medicine, 166, 1510-1524 (1897). Furthermore, aluminum salts may provoke transient or chronic local granulomas at the site of injection; L. H. Collier in lancet, 1354-1367 (1987) states that the incidence and severity of reactions to tetanus toxoid vaccine depends upon the presence of aluminum adjuvants. The preparation of aluminum adjuvants is not always reproducible. Moreover, aluminum can alone stimulate the production of IgE antibodies which are responsible for mediating immediate hypersensitive reactions. This has been described by T. Matuhasi et al, J. Infectious Disease, 146, 192 (1982).

Attention has focused in recent years on the use of organic compounds as immunoadjuvants. Only a few organic compounds function in a manner similar to commercially acceptable aluminum salts; i.e. as a slow release vehicle or antigen (vaccine) depot whereby antigen is released over a relatively long period of time at the site of injection.

Examples of such organic compounds are organic surfactants and emulsifiers, such as Pluronics and Tetronics which are non-ionic block copolymers of polyoxyethylene and polyoxypropylene produced by BASF Corporation. Such a slow-release mechanism of adjuvanticity has long been accepted for human use since it reduces the possibility of overstimulating the immune system. Overstimulation of the immune system can lead to an autoimmune response such as would occur with the use of a potent immunostimulant, for example Freund's adjuvant. Thus, the slow release mechanism is the preferred mechanism.

While the majority of organic adjuvants have been shown to be potent immunostimulants, such highly active adjuvants tend to be toxic and therefore unacceptable for human use. Examples of known organic adjuvants which are potent immunostimulants are Freund's complete adjuvant and muramyl dipeptide. Both of these compounds are restricted to use in animal research because of toxicity considerations. Many of the organic adjuvants which mimic aluminum salts are more toxic than aluminum salts. For example, long chain alkyl amines described by D. Gall in Immunology, 11, 369-386 (1966) are reported to be toxic compounds which are generally disruptive to cell membrane structure.

SUMMARY OF THE INVENTION

The present invention includes peptides of the formula (I):

    X-R.sub.1 -R.sub.2 -R.sub.3 -R.sub.4 -Y                    (I)

and pharmaceutically acceptable derivatives thereof, wherein;

X is selected from the group consisting of H, acetyl, and glycyl or a conservative substitution thereof.

R₁ is lysine or a conservative substitution thereof.

R₂ is asparagine or a conservative substitution thereof.

R₃ is proline or a conservative substitution thereof.

R₄ is tyrosine or a conservative substitution thereof.

Y is selected from the group consisting of OH, NH₂, and OC₁₋₆ alkyl.

In accordance with the present invention, there is provided peptides of formula (I) having an immunomodulatory activity.

In particular, the peptides of the present invention cause stimulation of the immune system in mammals including human.

In another aspect of the present invention, there is provided pharmaceutical compositions containing such immunomodulatory peptides of formula (I).

In a further aspect of the invention, there is provided the use of such peptides of formula (I) and compositions as izunomodulators, and, in particular, as immunostimulants.

In a further aspect of the invention, there is provided the use of such peptides of formula (I) and compositions for stimulating B-cells.

In a still further aspect of the invention, there is provided a peptide of formula (I) which acts as a control against tumor growth and viral infections.

Another aspect of the present invention is the use of the peptides of formula (I) or pharmaceutical preparations for the manufacture of a medicament for the treatment of immune deficiencies, in an amount effective to produce an immune response.

Another aspect of the invention is the method of treatment of a mammal, preferably a human, comprising the step of administering an effective amount peptide of formula I, a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof for the treatment of immune deficiency and tumor growth.

In a still further aspect of the invention, there is provided a peptide of formula (I) which can function as an adjuvant in a vaccine.

In a still further aspect of the invention, there is provided a peptide of formula (I) which can function as an adjuvant in a vaccine composition.

Another aspect of the present invention is the use of the peptides of formula (I) or pharmaceutical acceptable derivative for the manufacture of a vaccine composition.

Another aspect of the invention is the method for the prophylaxis of a disease, in a mammal, preferably a human, comprising the step of administering a vaccine comprising a compound of formula I, or a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A, 1B, 1C, and 1D show the activation of Ta and B cells in spleen in vivo/ex vivo by PEPTIDE #1 (SEQ. ID NO:1)

X is preferably selected from the group consisting of glycine; alanine; proline; glutamine; asparagine; serine; threonine; and valine. X is more preferably selected from the group consisting of glycine; alanine; and valine. X is most preferably hydrogen.

R₁ is preferably selected from the group consisting of D or L-lysine; arginine; ornithine; and histidine. R₁ is more preferably D-lysine or L-lysine. R₁ is most preferably D-lysine.

R₂ is preferably selected from the group consisting of D or L-asparagine; alanine; proline; glutamine; serine; threonine; valine; and glycine. R₂ is more preferably selected from the group consisting of D or L-asparagine; and glutamine. R₂ is most preferably asparagine.

In an alternative preferred embodiment, R₂ is glutamine.

R₃ is preferably selected from the group consisting of glycine; D or L-Proline; alanine; asparagine; glutamine; serine; threonine; valine; and glycine. R₃ is more preferably glycine or D or L-Proline. R₃ is most preferably D-Proline.

R₄ is preferably selected from the group consisting of D or L-tyrosine; cysteine; serine; threonine; phenylalanine; tryptophan; and histidine. R₄ is more preferably selected from the group consisting of D or L-tyrosine; and phenylalanine. R₄ is most preferably tyrosine.

In an alternative preferred embodiment, R₄ is phenylalanine. Y is preferably OH or NH₂. Y is most preferably OH.

Preferred peptides of the present invention are listed as follows:

PEPTIDE #1 H-Lys-Asn-Pro-Tyr-OH (SEQ. ID NO:1);

PEPTIDE #2 H-Lys-Asn-Gly-Tyr-OH (SEQ. ID NO:2);

PEPTIDE #3 H-Lys-Asn-(D-Pro)-Tyr-OH (SEQ. ID NO:1);

PEPTIDE #4 H-Lys-Asn-Pro-Phe-OH (SEQ. ID NO:3);

PEPTIDE #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ. ID NO:1);

PEPTIDE #6 H-Lys-Gln-Pro-Tyr-OH (SEQ. ID NO:4);

PEPTIDE #7 B-Gly-Lys-Asn-Pro-Tyr-OH (SEQ. ID NO:5);

PEPTIDE #8 H-Gly-Asn-Pro-Tyr-OH (SEQ. ID NO:6);

PEPTIDE #9 H-(D-Lys)-Asn-Gly-Tyr-OH (SEQ. ID NO:2);

PEPTIDE #10 H-(D-Lys)-Gln-Pro-Tyr-OH (SEQ. ID NO:4); and

PEPTIDE #11 H-(D-Lys)-Asn-Pro-Phe-OH (SEQ. ID NO:3).

The more preferred compound of the present invention are

PEPTIDE #1 H-Lys-Asn-Pro-Tyr-OH; (SEQ. ID NO:1) and

PEPTIDE #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ. ID NO:1).

The most preferred compound of the present invention is

PEPTIDE #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ. ID NO:1).

The term "amino acid" as employed herein includes and encompasses all of the naturally occurring amino acids, either in the D- or L-configuration if optically active, and the known non-native, synthetic, and modified amino acids, such as homocysteine, ornithine, norleucine and p-valine. A list of non natural amino acids may be found in "The Peptides", vol 5, 1983, Academic Press, Chapter 6 by D. C. Roberts and F. Vellaccio.

The term "conservative substitution" as employed herein refers to the amino acids in which the native sequence of the peptide of this invention have had conservative modifications or substitutions. These substitutions or modifications are those having a minimal influence on the secondary structure and hydropathic nature of the peptide. These include substitutions such as those described by Dayhoff in the Atlas of Protein Sequence and Structure 5, 1978 and in Argos in EMBO J. 8, 779-785, 1989. For example, amino acids belonging to one of the following groups represent conservative changes: ala, pro, gly, gln, asn, ser, thr, val; cys, ser, tyr, thr; val, ile, leu, met, ala, phe; lys, arg, orn, his; and phe, tyr, trp, his. The preferred substitutions also include substitutions of D-enantiomers for the corresponding L-amino acids.

The term "pharmaceutically acceptable derivative" as employed herein, includes any pharmaceutically acceptable salt, ester or amide, of a peptide of formula I or any other compound, analogue or derivative of formula 1 which, upon administration to the recipient, is capable of providing (directly or indirectly) a compound of formula I or an active metabolite or residue thereof.

The amino acids in the peptides of the present invention may be present in their natural L-configuration, unnatural D-configuration, or as a racemic mixture.

By the term "vaccine" is meant a preparation comprising killed microorganism, genitically modified microorganism, genitically engineered antigen (e.g. proteins, peptides, sugars, and/or glycopeptides), and/or naturally occuring antigen (e.g. proteins, peptides, sugars, and/or glycopeptides).

By the term "disease" is meant virus such as reovirus, rotavirus, orbivirus, retrovirus, lentivirus, picornavirus, papillomavirus, adenovirus, parvovirus, herpesvirus, poxvirus, hepadnavirus; and bacteria.

Virus can be selected from, but are not limited to the group consisting of influenza; hepatitis of type A, B, and C, human immune immunodefiency of type 1 and 2; chicken pox; cytomegalovirus; herpes virus of type 1 and 2; epstein barr; papilloma; and poliovirus of type I, II, and III.

Bacterium can be selected, but are not limited to the group consisting of tetanus; diptheria; pertussis; and meningococcus.

In a preferred embodiment, the adjuvant of this invention can be used in combination with the following vaccines: influenza; diphtheria, pertussis, tetanus(DPT); and meningitis.

There is also provided, in a further aspect of the invention a method for the prophylaxis of influenza, in a mammal, preferably a human, comprising the step of administering an influenza vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

There is also provided, in a further aspect of the invention a method for the prophylaxis of diphtheria, in a mammal, preferably a human, comprising the step of administering a diphtheria vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

There is also provided, in a further aspect of the invention a method for the prophylaxis of pertussis, in a mammal, preferably a human, comprising the step of administering a pertussis vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

There is also provided, in a further aspect of the invention a method for the prophylaxis of tetanus, in a mammal, preferably a human, comprising the step of administering a tetanus vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

There is also provided, in a further aspect of the invention a method for the prophylaxis of diphtheria, pertussis, and tetanus(DPT), in a mammal, preferably a human, comprising the step of administering a DPT vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

There is also provided, in a further aspect of the invention, a method for the prophylaxis of meningitis, in a mammal, preferably a human, comprising the step of administering a meningitis vaccine comprising a peptide of formula (I), a pharmaceutical composition, or a pharmaceutically acceptable derivative thereof, in an amount effective to produce an immune response.

It will be appreciated by those skilled in the art that the reference herein to treatment extends to prophylaxis as well as treatment of established infections or symptoms and therefore includes control of tumor outgrowth.

It will be further appreciated that the amount of a compound of the invention required for use in treatment will vary not only with the particular compound selected but also with the route of administration, the nature of the condition being treated, and the age and condition of the patient, and will be ultimately at the discretion of the attendant physician or veterinarian. In general, however, a suitable dose will be in the range from about 0.1 to about 750 mg/kg of body weight per day.

The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example as two, three, four or more sub-doses per day.

The compound is conveniently administered in unit dosage form; for example containing 10 to 1500 mg, of active ingredient per unit dosage form.

Ideally the active ingredient should be administered to achieve peak plasma concentrations of the active compound. This may be achieved, for example, by the intravenous injection of a solution of the active ingredient, optionally in saline, or administered as a bolus. Desirable blood levels may be maintained by a continuous infusion or by intermitent infusions.

While it is possible that, for use in therapy, a compound of the invention may be administered as the raw chemical, it is preferable to present the active ingredient as a pharmaceutical formulation.

The invention thus further provides a pharmaceutical formulation comprising a compound of formula (I) or a pharmaceutically acceptable derivative thereof together with one or more pharmaceutically acceptable carriers thereof and, optionally, other therapeutic ingredients. The carrier(s) must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

Pharmaceutical formulations include those suitable for oral or parenteral (including intramuscular, sub-cutaneous and intravenous) administration. The formulations may, where appropriate, be conveniently presented in discrete dosage units and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association the active compound with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.

When desired, the above described formulations adapted to give sustained release of the active ingredient may be employed.

The peptides of the invention may also be used in combination with other therapeutically active agents, for example, other immunomodulators, antiviral agents, or anticancer agents.

The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a combination as defined above together with a pharmaceutically acceptable carrier thereof comprise a further aspect of the invention.

The individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.

When the peptide of formula (I) or a pharmaceutically acceptable derivative thereof is used in combination with a second therapeutic agent, the dose of each compound may be either the same or differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.

It will be appreciated by those skilled in the art that the peptides of the present invention include all pharmaceutically acceptable derivatives and analogues thereof, as well as all isomers and enantiomers.

The vaccine compositions comprising the adjuvant of the invention can be prepared by physically mixing the adjuvant with the appropriate antigen under appropriate sterile conditions in accordance with known techniques to produce the adjuvanted compositions.

The amounts of the adjuvant and antigen needed to elicit an immune response in humans are interrelated, but are within the ranges generally employed in conventional vaccines. For example, the use of increasing amounts of adjuvant may suggest that decreasing amounts of antigen can be used, and vice versa. The preferred amount of adjuvant is 0.01 to 5 mg/ml of the composition, for example 0.05 mg/ml to 3 mg/ml, preferably 0.5 to 1.0 mg/ml. The preferred amount-of antigen is between about 1 to 100 micrograms/ml. Preferably about 5 to 45 micrograms/ml. The dosage will depend upon the host receiving the vaccine as well as factors such as the size, weight, and age of the host.

The vaccine compositions comprisimg the adjuvant of this invention may be formulated using techniques similar to those used for other pharmaceutical compositions. Thus, the adjuvant and antigen may be stored in lyophilized form and reconstituted in a physiologically acceptable vehicle to form a mixture prior to administration. Alternatively, the adjuvant and antigen may be stored in the vehicle. Preferred vehicles are sterile solutions, in particular, sterile buffer solutions, such as phosphate buffered saline. Any method of combining the adjuvant and the antigen in the vehicle such that improved immunological effectiveness of the composition relative to the individual components is appropriate.

The vehicle may contain preservatives or other known additives which are used to improve the shelf stability or the efficacy of the mixture. Suitable preservatives include, for example, thimerosal.

The vaccine compositions comprising the adjuvant of the invention may be administered by any convenient means. Preferred methods of administration include subcutaneous, intramuscular, intradermal, or by way of oral or nasal delivery. Alternatively, the mixture may be released from a biodiffusible implant. A single adminisration may be used. Alternatively a series of administrations may be made over the course of several days or weeks.

The peptides of formula (I) are prepared using standard solid phase peptide synthesis procedures. These procedures are well known to those persons skilled in the art. Such procedures are described in John M. Stewart and Janis D. Young, Solid Phase Peptide Synthesis, 2nd ed., (1984) Pierce Chemical Co., Illinois, U.S., which is incorporated herein by reference.

EXAMPLES

The peptides of formula I were synthesized and tested for immunological activity using the procedures outlined below. The following examples are not intended to limit the invention in any way.

Example 1 Synthesis Protocol for the Peptides of the Invention

abbreviations:

Pmoc: Fluorenylmethoxycarbonyl;

DMF: Dimethylformamide;

TFA: Trifluoroacetic acid;

HPLC: High performance liquid chromatography;

HOBT: 1-Hydroxybenzotriazole;

DCC: Dicylohexylcarbodiimide;

t-Bu: Tert-butyl;

Boc: Butoxycarbonyl;

Trt: Trityl.

The synthetic peptide was prepared using p-benzyloxybenzyl alcohol resin functionalized with the relevant C-terminal N-protected Fmoc-amino acid [Fmoc-Tyrosine(tBu)] and capped with benzyl chloride.

The remaining amino acids were all Fmoc protected at the alpha amino group and the following side chain protecting groups were utilized: Trt for asparagine and Boc for lysine.

Reagents used in the synthesis and cleavage were all ACS grade and used as such without further purification with the exception of the DMF used for the couplings which was dimethylamine free and TFA used in the cleavage which was of biograde purity. H₂ O and acetonitrile used for the purification were HPLC grade solvents. HCl used for salt exchange was of constant boiling grade.

Solid phase peptide synthesis was carried out manually. Resin loading was in the order of 0.53 meq/g and synthesis was carried out on a 10.59 mM scale.

Peptide condensation was carried out using 2 equivalents of Fmoc amino acid, HOBT and DCC in DMF (dimethylamine free) for 2 hours to overnight couplings at room temperature. Recouplings were carried out using 1 equivalent of reagents for 2-4 hours. Deprotection of the Fmoc moiety was accomplished using 20% (v/v) piperidine in DMF for 20-25 minutes at room temperature.

Cleavage of the peptide from the resin and.removal of the side chain protecting groups (tBu, Boc, Trt) was carried out using a solution containing 55% TFA, 5% anisole and 40% dichloromethane for 90 minutes at room temperature under a nitrogen atmosphere. The resin was then washed successively using a solution of 20% TFA, 0.5% anisole and 79.5% dichloromethane. The combined filtrates were then evaporated under reduced pressure and the peptide was precipitated from diethyl ether, filtered, and lyophilized. The crude peptide was purified by HPLC on a preparative reversed phase column (Vydac® C18, 300 Å, 10-15 microns) using a flow rate of 160 ml/min with a gradient using H₂ O plus 0.06% TFA and acetonitrile plus 0.06% TFA in a 0-60% gradient over 60 minutes. Salt exchange to the HCl form was accomplished with the addition of an excess amount of HCl to the peptide in solution with successive lyophilization cycles.

The amino acid content of the purified tetrapeptide was determined by amino acid analysis after hydrolysis; 6N HCl, 110 C for 20 hours. A typical amino acid analysis is represented by the following:

Lys=1.02; Asp/Asn=0.99; Pro=1.07; Tyr=1.00.

Peptides synthesized by this method were tested in vitro and in vivo to assess imunomodulatory activity in the following manner.

Example 2 In Virto Stimulation of Immune Cell Subsets

The following abbreviations and definitions are used herein:

PHA--Phytohemagglutinin

ConA--Concanavalin A

LPS--Lipopolysaccharide

PMW--Pokeweed mitogen

PBS--Phosphate buffered saline

1. Cytotoxic T Lymphocytes (CTL) Mixed Lymphocyte Reaction (MLR) and Mitogenic Response Assays

The protocols used are described in Hudson L., Haay F., Pratical immunology, 3^(rd) edition, Blackwell, Scientific publications, Oxford 1989, p. 158-162, and p.447.

Lymphocytes were isolated from C57BL/6 mice and DBA/2 mice in accordance to techniques well known in the art. Cell concentration was adjusted to 10×10⁶ cell/ml for each assay. The cells isolated from DBA/2 mice were irradiated with 3000 Rads.

In a 6 well plate, each well was set up with:

1 ml of C57BL/6 cells;

1 ml of DBA/2 cells; and

1 ml of the drug at different concentrations (or IL-2 to 10 ng/ml final) in RPMI 10% supplemented.

The cells were incubated for five days at 37° C. in 5% CO₂, after which CTL and MLR assays were performed.

Results are presented in Table 1.

a) Cytotoxic T Lymphocytes (CTL)

P815 target cells were labelled with Cr⁵¹ (0.1 mCi). After labelling, the cells were washed three times and resuspended to a concentration of 5×10⁴ cells/ml in media. p T cells were then counted and diluted to obtain a concentration of 2.5×10⁶ cells/ml, and then further diluted to obtain the following effector:target ratios:

50:1 (2.5×10⁶ cells/ml: 5×10⁴ cell/ml)

25:1 (1.25×10⁶ cells/ml: 5×10⁴ cells/ml)

12.5:1 (0.625×10⁶ cells/ml: 5×10⁴ cells/ml).

100 μl of target cells+100 μl of CTLs were incubated for 4 hours. In parallel wells, 100 μl of target cells+100 μl of media were incubated for spntaneous release. After incubation, cells were centrifuged and 100 μl of supernatant was then counted using a gamma counter.

Results are expressed as % specific ⁵¹ Cr release= ##EQU1## where ER=experimental release, SR=spontaneous releaase, and

TR=total release.

b) Mixed Lymphocyte Reaction (MLR)

Mixed lymphocyte reaction is an in vitro counterpart of the allograft rejection. Briefly, lymphocyte activation (mitotic response) is obtained when cells from two inbred strains or from two outbred individuals of any species are mixed in in vitro culture. To have a unidirectional response, the proliferation of either cell type may be blocked with X-ray irradiation or mitomycin C treatment. After 4 days incubation, ³ H thymidine uptake and cytotoxicity assays (CTL) were performed. The cells were then resuspended and 100 μl of cell suspension was deposited in each well of a 96 well plate. 1 μCi of tritiated thymidine was added for 6 hours. After incubation, the cells were then harvested and counted using a Microbeta counter.

c) Activation of T or B cells by Mitogenic Proliferation

A mitogenic lectin (mitogen) is a glycoprotein which binds and cross-links specific cells surface carbohydrate determinants, and will polyclonally stimulate lymphoid cells. Lymphocyte activation by either antigens or mitogens results in intracellular changes and the subsequent development into a lymphoblast. Mitogenic stimulation of lymphocytes in vitro is believed to mimic the series of events which occur in vivo following their stimulation by specific antigens. PHA and ConA; PWM and LPS mitogens can be used as a measurement of T cell and B cell activity, respectively. Briefly, the spleen mononuclear leukocytes from C57BL/6 mice are incubated in the presence or absence of mitogens with or without candidate drugs. After 72 hours or 5 days, ³ H-thymidine incorporation is recorded as an indication of cell proliferation.

Lymphocytes were isolated from mice spleen or human blood in accordance to methods well known in the art. 100 μl of cell suspension of 2×10⁶ cells/ml was added per well (2x10⁵ cells/well) and incubated in the presence of lectin at the following concentrations:

Human T cells:

PHA=0.01% final concentration

ConA=0.2 μg/ml

Human B cells:

PWM=0.01 to 0.1 μg/ml

LPS=10 μg/ml

Mouse T cells:

PHA=0.01% to 0.001% final concentration

ConA=1 to 2 μg/ml

Mouse B cells:

PWM=0.2 to 0.02% final concentration

LPS=2 to 5 μg/ml

Cells were incubated for 3 days with PHA or ConA, or 5 days with PWM or LPS. 1 μCi of tritiated thymidine was added to each well for the last 6 hours (or 0.5 μCi for 18 hrs). The cells were harvested in a cell Harvester (Tortech®) and counted in a Beta counter.

For CTL activity, the data expressed is as a % augmentation compared to IL-2. IL-2 is 100%. 0 represents less than 20%, + represents 20-40%, ++ represents 40-60%, +++ represent 60-80%, and ++++ represents 80% and over.

Table 1 shows that peptide #1 (SEQ. ID NO:1) stimulates CTL's and B cells in vitro. Peptide #2 (SEQ. ID NO:2) stimulates T cells to a moderate extent and stimulates B cells. Peptide #3 (SEQ. ID NO:1) can be seen to stimulate CTL.

After in vitro drug analysis, the drugs are evaluated in whole blood for drug stability and toxicity.

                                      TABLE 1                                      __________________________________________________________________________     Activation of CTL/MLR Activation of T Cells                                                                        Activation of B Cells                      Compounds                                                                            CTL     MLR     ConA       PHA                                                                               LPS        PWM                             __________________________________________________________________________     #1    ++(10.sup.-7 M)                                                                        0       0          0  1.9 - 3.6 × (10.sup.-10 -                                                10.sup.-4  1.7 - 4 × (10.sup.-6                                                     - 10.sup.-4 M)                         M)                                                                        #2 ++++(10.sup.-9 M) 1.8 × (10.sup.-9 M) 1.3 × (10.sup.-12                                                      M - 10.sup.-10 0 1.8 - 3.5                                                     × (10.sup.-8 -                                                           10.sup.-5 1.4 - 4.5                                                            × (10.sup.-8 -                                                           10.sup.-5                            M)  M) M)                                                                   #3 ++(10.sup.-9 M) 0 0 0 0 0                                                   #4 0 0 0 0 0 0                                                                 #5 ++++(10.sup.-9 M) 2.1 × (10.sup.-7 M) 0 0 0 0                         LANT-6 +++(10.sup.-7 M) 0 0 0 0 0                                              #6 0 0 0 0 0 0                                                                 #7 N.D. N.D. 0 0 0 0                                                           #8 N.D. N.D. 0 0 0 0                                                         __________________________________________________________________________      N.D. = not determined                                                    

Example 3 In vivo/ex vivo Stimulation of Immune Cell Subsets

C57BL\6 mice are treated with 0, 50, 100, 200, and 300 mg/kg of drug (in saline) for 4 consecutive days. Blood is taken by cardiac puncture on day 5, and the lymphocytes are isolated from spleen in the following manner:

The spleen is aseptically removed and mashed by pressing against a steel strainer with a rubber plunger. The cell suspension is separated from the clumps by letting them settle at the bottom of the tube. The cell suspension is then separated on a Lymphocyte M layer and the interface is recovered and resuspended in media.

The following assays are performed:

1. Immunophenotyping (blood and spleen)

Cell immunophenotyping is performed on blood and spleen. The following cell surface antigens are analyzed:

CD3 (all T cells);

CD4 (T helper/inducer, binds class II-restricted T cells);

CD8 (cytotoxic T cells, CTL adhesion);

MAC-1 (monocyte/macrophage);

NK (natural killer cells);

Ly5 (CD20) (B cells);

CD45 (all leukocytes, protein tyrosine phosphatase).

Peptide #1 (SEQ. ID NO:1) was tested in one assay, the results are reported in table 1A, 2B, 3A, and 3B. In the spleen, a significant increase of relative percentage of NK+CD3+ (NK cells) and CD8+CD45+ (cytotoxic T cells) subsets was observed at 200 and 300 mg/kg for Peptide #1 (SEQ. ID NO:1).

Peptide #5 (SEQ. ID NO:1) was tested in two assays, the results of the first assay are reported in table 4A, 4B, 5A, and 5B, the results of the second assay are reported in table 6A, 6B, 7A, and 7B. On blood immunophenotyping, Peptide #5 (SEQ. ID NO:1) reduced in a significant manner the relative percentage of T cells which is shown by a reduction in TCR, CD4+ and CD8+ cell surface antigen. In a similar fashion, a significant increase of B cells is observed (Ly5 (80% CD4- CD8- (73%)) at all tested doses.

                                      TABLE 2A                                     __________________________________________________________________________     Immunophenotyping of cell subset CD45-CD4-CD8 of COMPOUND #1 in the blood          CELL SUBSET                                                                DOSE                                                                               CD4 +                                                                              CD4 +                                                                              CD4 -                                                                              CD4 -                                                                              CD4 -                                                                              CD4 +                                                                              CD4 -                                                                              CD4 +                                                                              CD8 +                                                                              CD8 +                                                                              CD8 -                                                                              CD8 -                            mg/kg CD45- CD45+ CD45- CD45+ CD8+ CD8+ CD8- CD8- CD45- CD45+ CD45-                                                          CD45+                          __________________________________________________________________________     CTL 3.53                                                                               31.23                                                                              9.27                                                                               55.97                                                                              12.90                                                                              2.97                                                                               52.33                                                                              31.83                                                                              0.33                                                                               15.03                                                                              15.03                                                                              69.60                            (S.D.) 0.40 8.89 3.46 7.43 6.51 2.25 15.41 7.01 0.58 8.49 3.04 7.20                                                           50 3.80 26.95 3.90 65.30                                                      11.20 3.65 58.05 27.15                                                         0.55 13.70 9.60 76.15                                                           (S.D.) 0.14 1.34 0.42                                                         0.71 1.56 2.19 2.76 1.06                                                       0.35 3.39 0.85 2.90                                                             P    0.28 0.38 0.10 0.43                                                        0.35 0.04 0.31                 100 8.90 46.75 7.00 37.30 11.50 27.20 32.85 28.55 6.20 31.95 14.35                                                           47.55                            (S.D.) 8.63 17.89 3.54 29.98 1.70 37.34 28.21 10.82 8.77 30.19 6.01                                                          44.90                            P    0.34 0.39 0.25 0.32   0.31 0.28 0.36                                      200 6.22 33.01 6.74 40.63 8.57 16.11 37.41 24.11 4.13 20.68 10.39 49.80        (S.D.) 3.58 12.79 5.55 25.47 5.40 19.44 22.28 9.23 4.67 16.52 7.29                                                           31.67                            P    0.44 0.43 0.25 0.44   0.31 0.43 0.35                                      300 2.77 30.67 4.47 62.10 16.67 2.37 49.90 31.07 -- 18.10 8.83 73.03                                                          (S.D.) 1.08 8.31 2.48                                                         11.09 1.50 1.95 10.76                                                          7.76 -- 3.24 3.41 6.18                                                          P    0.20 0.19 0.39 0.42                                                        0.31 0.06 0.17               __________________________________________________________________________

                                      TABLE 2B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8, B and CD4-CD11 of COMPOUND #1 in      the blood                                                                          CELL SUBSET                                                                DOSE                                                                               NK +                                                                               NK +            Non   CD4 +                                                                              CD4 +                                                                              CD4 -                                                                              CD4 -                                  mg/kg CD3- CD3+ NK - CD3- NK - CD3+ B B CD11- CD11+ CD11- CD11+              __________________________________________________________________________     CTL 8.03                                                                               5.47                                                                               45.43 41.07 78.80                                                                             21.20                                                                             30.63                                                                              0.33                                                                               63.87                                                                              5.23                                   (S.D.) 2.50 5.40 17.50 9.70 3.98 3.98 6.21 0.15 7.52 1.60                      50 10.47 7.83 40.53 41.23 76.40 23.57 34.10 0.53 57/73 7.67                    (S.D.) 2.22 3.59 12.19 8.33 4.41 4.41 7.83 0.32 9.67 2.32                      P 0.13 0.24    0.12                                                            100 6.50 2.80 53.13 37.57 80.77 19.23 41.67 0.23 55.13 3.00                    (S.D.) 4.06 1.37 9.60 5.23 14.66 14.66 29.59 0.06 27.76 1.93                   P 0.28 0.24    0.41                                                            200 8.70 4.57 55.07 31.73 79.90 20.10 39.73 0.50 54.30 5.50                    (S.D.) 3.93 2.21 15.61 9.55 10.56 10.56 21.50 0.26 18.40 4.49                  P 0.33 0.35    0.43                                                            300 11.87 9.97 35.77 42.43 66.30 33.67 29.53 0.37 65.03 5.07                   (S.D.) 2.32 5.59 11.46 4.76 1.39 1.42 2.87 0.15 3.67 0.97                      P 0.13 0.26    0.02                                                          __________________________________________________________________________      In the blood, a significant increase of the relative percentage of B cell      was observed at 300 mg/kg of compound #1                                 

                                      TABLE 3A                                     __________________________________________________________________________     Immunophenotyping in cell subset CD45-CD4-CD8 of COMPOUND #1 in the            spleen                                                                              CELL SUBSET                                                               DOSE CD4 +                                                                              CD4 +                                                                              CD4 -                                                                               CD4 -                                                                              CD4 -                                                                               CD4 +                                                                              CD4 -                                                                              CD4 +                                                                              CD8 +                                                                              CD8 +                                                                              CD8 -                                                                              CD8 -   CD45 -                                                                          mg/kg CD45-                                                                   CD45+ CD45 -                                                                   CD45+ CD8+                                                                     CD8+ CD8- CD8-                                                                 CD45- CD45+                                                                    CD45- CD45+                                                                    CD45-8              __________________________________________________________________________                                                                Th1                 CTL  4.63                                                                               30.47                                                                              1.63 63.33                                                                              11.67                                                                               1.97                                                                               53.27                                                                              33.10                                                                              0.07                                                                               13.03                                                                              7.77                                                                               79.17                                                                              62.70                                                                              37.35                 (S.D.) 1.02 4.55 0.15 4.70 1.80 1.16 5.80 3.74 0.06 2.84 0.99 2.58 1.84                                                                 1.77                  50 6.10 33.30 1.95 58.65 16.15 1.10 44.45 38.30 0.10 16.90 9.85 73.15                                                                   60.70 39.10                                                                     (S.D.) 0.14                                                                   1.27 0.78 2.33                                                                 1.91 0.14 0.49                                                                 1.56 0.00 2.12                                                                 1.48 0.64 1.41                                                                 1.41                  P    0.10      0.16                                                            100 3.80 30.90 3.85 61.45 12.45 2.40 52.85 32.30 0.05 14.25 9.35 76.35                                                                  65.55 34.55                                                                     (S.D.) 0.71                                                                   1.39 2.33 1.77                                                                 1.48 0.42 5.59                                                                 3.68 0.07 1.91                                                                 1.34 0.64 4.17                                                                 4.17                  P    0.33      0.43                                                            200 3.77 27.98 2.31 43.44 12.49 2.27 40.94 29.50 0.04 12.36 7.32 62.30                                                                  53.14 31.64                                                                     (S.D.) 1.55                                                                   12.30 0.81                                                                     29.04 6.16                                                                     1.46 18.14                                                                     13.03 0.04                                                                     8.40 2.96                                                                      30.44 24.16                                                                    13.76                 P    0.28      0.08                                                            300 3.67 35.00 3.97 57.40 16.17 4.33 45.20 34.30 0.03 19.77 10.30 69.93                                                                 61.63 38.43                                                                     (S.D.) 1.93                                                                   5.26 1.39 4.69                                                                 0.90 2.66 6.47                                                                 5.67 0.06 2.02                                                                 0.80 1.48 5.84                                                                 5.90                  P    0.11      0.00                                                          __________________________________________________________________________

                                      TABLE 3B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8,B and CD4-CD11 of COMPOUND #1 in       the spleen                                                                         CELL SUBSET                                                                DOSE                                                                               NK +                                                                               NK +               CD4 +                                                                              CD4 +                                                                              CD4 -                                                                              CD4 -                                     mg/kg CD3- CD3+ NK - CD3- NK - CD3+ B CD11- CD11+ CD11- CD11+                __________________________________________________________________________     CTL 8.17                                                                               4.07                                                                               49.17 38.63 13.07                                                                             22.87                                                                              0.13                                                                               75.67                                                                              1.37                                      (S.D.) 0.61 1.88 10.30 8.80 1.46 0.80 0.06 1.11 0.31                           50 7.47 4.70 45.10 42.80 11.60 18.47 0.17 80.17 1.20                           (S.D.) 0.64 0.72 2.40 2.72 3.00 0.90 0.06 0.55 0.46                            P 0.05 0.27 0.23 0.18 0.26 0.02                                                100 7.93 4.40 46.20 41.50 14.97 18.23 0.20 80.07 1.53                          (S.D.) 2.31 1.37 0.82 3.03 0.40 1.67 0.10 1.86 0.21                            P 0.43 0.44 0.34 0.26 0.09 0.03                                                200 8.07 5.27 42.33 44.33 16.33 20.03 0.20 78.27 1.53                          (S.D.) 1.04 1.14 1.61 3.70 2.42 1.15 0.26 0.74 0.21                            P 0.46 0.06 0.21 0.25 0.06 0.06                                                300 10.83 9.47 38.63 41.10 14.60 20.53 0.53 76.67 2.23                         (S.D.) 0.86 3.52 6.85 2.66 5.36 2.63 0.15 2.80 0.12                            p 0.01 0.05 0.07 0.31 0.31 0.08                                              __________________________________________________________________________

                                      TABLE 4A                                     __________________________________________________________________________     Immunophenotyping of cell subset CD45-CD4-CD8 of COMPOUND #5 in the blood          CD4 +                                                                              CD4 +                                                                              CD4 -                                                                              CD4 -                                                                              CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                             CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                           CD8- CD8+ CD8- CD8+ CD4- CD4+ CD4- CD4+ CD8- CD8+ CD8- CDE8+                 __________________________________________________________________________     CTL                                                                              MEAN 25.78 3.10 62.20 8.95 51.60 24.73 18.30 5.38 56.15 10.70 31.80                                                          1.33                             S-D 2.97 0.35 3.24 1.47 4.22 5.71 7.41 1.45 6.94 1.25 5.60 0.95              COMPOUND #5 - 50 mg/kg                                                         MEAN                                                                               18.15                                                                              2.03                                                                               74.68                                                                              5.15                                                                               57.68                                                                              17.00                                                                              22.15                                                                              3.15                                                                               62.48                                                                              6.53                                                                               30.35                                                                              0.68                             S-D 3.34 0.33 4.29 1.07 2.69 2.99 4.69 0.45 3.71 1.25 3.61 0.13                                                               P 0.000 0.005 0.002                                                           0.012 0.019 0.006 0.093                                                        0.042 0.033 0.004 0.213                                                        0.156                          COMPOUND #5 - 100 mg/kg                                                        MEAN                                                                               17.90                                                                              2.10                                                                               73.95                                                                              6.03                                                                               58.75                                                                              16.78                                                                              21.23                                                                              3.25                                                                               63.45                                                                              7.10                                                                               28.38                                                                              1.03                             S-D 1.99 0.79 3.91 1.23 1.66 2.57 3.36 0.37 1.97 1.87 3.74 0.25                                                               P 0.008 0.019 0.004                                                           0.001 0.043 0.041 0.297                                                        0.031 0.094 0.007 0.253                                                        0.274                          COMPOUND #5 - 200 mg/kg                                                        MEAN                                                                               19.00                                                                              1.90                                                                               74.40                                                                              4.68                                                                               57.70                                                                              17.45                                                                              21.35                                                                              3.48                                                                               64.30                                                                              6.15                                                                               29.08                                                                              0.48                             S-D 3.58 0.86 6.01 1.83 4.44 3.71 7.60 0.66 5.06 2.54 7.03 0.13                                                               P 0.017 0.022 0.008                                                           0.002 0.114 0.069 0.314                                                        0.045 0.118 0.012 0.334                                                        0.082                          __________________________________________________________________________

                                      TABLE 4B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8, T.B and CD4-CD11 of COMPOUND#5        in the blood                                                                                                                       CD4 -                                                                               CD4 -                   NK + CD3- NK + CD3+ NK - CD3- NK - CD3+ TCR(T) Ly5(B) CD4 + CD11b- CD4                                                                + CD11b+ CD11b-                                                                CDllb+                __________________________________________________________________________     CTL                                                                              MEAN 5.28 3.08 50.35 41.33 41.10 36.65 27.90 2.23 52.50 17.35                  S-D 1.60 0.95 3.39 2.85 3.85 14.35 2.49 1.31 9.84 6.70                       COMPOUND #5 - 50 mg/kg                                                         MEAN                                                                               5.70  2.13  58.93 33.30 31.53                                                                              57.88                                                                              22.55   1.18    64.15                                                                               12.08                   S-D 0.64 0.73 7.06 6.71 5.47 9.89 3.90 0.91 7.42 3.88                          P 0.334 0.106 0.068 0.045 0.022 0.016 0.066 0.105 0.076 0.102                COMPOUND #5 - 100 mg/kg                                                        MEAN                                                                               6.00  2.43  57.95 33.58 32.23                                                                              58.73                                                                              20.30   1.10    67.65                                                                               11.03                   S-D 0.75 0.62 0.98 1.28 1.69 6.19 0.41 0.67 4.71 3.89                          P 0.273 0.222 0.007 0.001 0.004 0.059 0.003 0.162 0.053 0.140                COMPOUND #5 200 mg/kg                                                          MEAN                                                                               5.73  1.93  59.33 33.03 32.18                                                                              65.60                                                                              18.98   0.85    72.45                                                                               7.78                    S-D 0.90 0.36 4.15 3.90 6.14 3.40 4.30 0.17 3.93 0.83                          P 0.362 0.023 0.001 0.007 0.027 0.014 0.027 0.063 0.015 0.028                __________________________________________________________________________

                                      TABLE 5A                                     __________________________________________________________________________     Immunophenotyping of cell subset CD45-CD4-CD8 of COMPOUND #5 in the            spleen                                                                                                  CD4 - CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                             CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                          CD4 + CD8-                                                                    CD4 + CD8+ CD4                                                                 - CD8- CD8+                                                                    CD4- CD4+ CD4-                                                                 CD4+ CD8- CD8+                                                                 CD8- CD8+           __________________________________________________________________________     CTL                                                                              MEAN 33.75 0.60 59.10 6.53 55.68 21.75 8.95 13.65 66.33 6.10 26.90 0.68        S-D 4.56 0.14 5.14 0.48 5.14 3.96 0.48 0.89 2.73 0.56 2.12 0.10              COMPOUND #5 - 50 mg/kg                                                         MEAN                                                                               33.05  0.58   60.65  5.73  55.25                                                                              21.43                                                                              10.03                                                                              13.28                                                                              66.00                                                                              5.38                                                                               28.05                                                                              0.63                  S-D 1.29 0.05 1.87 0.68 1.42 1.21 1.03 0.68 1.97 0.51 1.42 0.17                                                                          P 0.360 0.380                                                                 0.226 0.015                                                                    0.427 0.430                                                                    0.070 0.029                                                                    0.372 0.016                                                                    0.124 0.302         COMPOUND #5 100 mg/kg                                                          MEAN                                                                               36.85  0.70   55.50  7.03  52.10                                                                              23.25                                                                              9.30                                                                               15.35                                                                              63.43                                                                              6.43                                                                               29.30                                                                              0.83                  S-D 1.25 0.26 1.89 0.54 1.50 1.58 0.64 1.18 1.90 0.82 1.78 0.05                                                                          P 0.139 0.177                                                                 0.122 0.050                                                                    0.127 0.222                                                                    0.179 0.054                                                                    0.064 0.189                                                                    0.081 0.007         COMPOUND #5 200 mg/kg                                                          MEAN                                                                               33.73  0.63   60.00  5.68  54.03                                                                              21.18                                                                              10.48                                                                              14.33                                                                              64.83                                                                              5.28                                                                               29.35                                                                              0.55                  S-D 2.45 0.13 3.09 0.56 3.37 1.60 1.09 1.00 2.60 0.62 2.15 0.06                                                                          P 0.497 0.427                                                                 0.417 0.085                                                                    0.361 0.421                                                                    0.069 0.262                                                                    0.306 0.111                                                                    0.163 0.071         __________________________________________________________________________

                                      TABLE 5B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8, T.B and CD4-CD11 of COMPOUND #5       in the spleen                                                                                                                      CD4 -                                                                               CD4 -                   NK + CD3- NK + CD3+ NK - CD3- NK - CD3+ TCR(T) Ly5(B) CD4 + CD11b- CD4                                                                + CD11b+ CD11b-                                                                CDllb+                __________________________________________________________________________     CTL                                                                              MEAN 3.55 1.68 57.58 37.20 38.55 58.53 25.73 0.78 70.13 3.40                   S-D 0.17 0.21 3.51 3.40 3.14 3.91 2.04 0.05 2.60 0.71                        COMPOUND #5 50 mg/kg                                                           MEAN                                                                               3.78  1.50  56.68 38.13 40.23                                                                              57.85                                                                              23.88   0.83    71.73                                                                               3.63                    S-D 0.28 0.18 1.19 1.40 1.10 1.69 0.70 0.10 0.88 0.24                          P 0.039 0.094 0.325 0.326 0.199 0.380 0.044 0.196 0.117 0.292                COMPOUND #5 100 mg/kg                                                          MEAN                                                                               3.88  1.83  55.95 38.38 39.83                                                                              56.75                                                                              23.73   0.95    71.23                                                                               4.10                    S-D 0.32 0.13 1.62 1.51 1.90 1.47 1.25 0.06 1.33 0.34                          P 0.137 0.091 0.124 0.217 0.190 0.160 0.020 0.018 0.096 0.094                COMPOUND #5 200 mg/kg                                                          MEAN                                                                               3.88  1.95  58.50 35.70 37.30                                                                              59.50                                                                              22.65   1.00    72.00                                                                               3.58                    S-D 0.22 0.25 2.23 2.27 1.94 1.49 1.31 0.22 1.54 0.28                          P 0.016 0.112 0.379 0.303 0.313 0.370 0.071 0.068 0.129 0.285                __________________________________________________________________________

                                      TABLE 6A                                     __________________________________________________________________________     Immunophenotyping of cell subset CD45-CD4-CD8 of COMPOUND #5 in the blood                               CD4 - CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                             CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                          CD4 + CD8-                                                                    CD4 + CD8+ CD4                                                                 - CD8- CD8+                                                                    CD4- CD4+ CD4-                                                                 CD4+ CD8- CD8+                                                                 CD8- CD8+           __________________________________________________________________________     CTL                                                                              MEAN 25.85 0.58 62.70 7.23 65.80 24.45 8.00 1.78 83.45 7.73 8.75 0.08                                                                    S-D 2.25 0.13                                                                 3.15 2.11 4.78                                                                 1.95 3.09 0.38                                                                 3.04 2.20 3.33                                                                 0.05                COMPOUND #5 - 50 mg/kg                                                         MEAN                                                                               20.65  0.43   71.20  7.70  68.23                                                                              18.48                                                                              11.05                                                                              2.25                                                                               79.70                                                                              8.10                                                                               12.15                                                                              0.03                  S-D 2.38 0.15 2.46 1.41 5.37 2.46 6.42 0.47 5.58 1.54 6.28 0.05                                                                          P 0.025 0.148                                                                 0.017 0.383                                                                    0.306 0.014                                                                    0.219 0.157                                                                    0.127 0.413                                                                    0.206 0.091         COMPOUND #5 - 100 mg/kg                                                        MEAN                                                                               25.00  0.33   67.83  6.80  68.45                                                                              22.70                                                                              6.63                                                                               2.23                                                                               85.00                                                                              7.05                                                                               7.83                                                                               0.10                  S-D 5.57 0.21 6.91 1.93 7.19 5.09 2.35 0.49 4.58 2.09 2.52 0.08                                                                          P 0.413 0.078                                                                 0.390 0.397                                                                    0.335 0.314                                                                    0.286 0.186                                                                    0.345 0.352                                                                    0.365 0.196         COMPOUND #5 200 mg/kg                                                          MEAN                                                                               23.13  0.30   69.03  7.58  71.95                                                                              20.85                                                                              5.00                                                                               2.23                                                                               85.60                                                                              7.83                                                                               6.53                                                                               0.05                  S-D 4.28 0.08 4.80 0.82 5.51 4.32 1.34 0.35 1.61 0.88 1.34 0.06                                                                          P 0.205 0.011                                                                 0.229 0.330                                                                    0.093 0.140                                                                    0.053 0.007                                                                    0.111 0.451                                                                    0.099 0.319         __________________________________________________________________________

                                      TABLE 6B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8, T.B and CD4-CD11 of COMPOUND #5       in the blood                                                                                                                       CD4 -                                                                               CD4 -                   NK + CD3- NK + CD3+ NK - CD3- NK - CD3+ TCR(T) Ly5(B) CD4 + CD11b- CD4                                                                + CD11b+ CD11b-                                                                CDllb+                __________________________________________________________________________     CTL                                                                              MEAN 7.05 3.58 50.43 38.95 42.33 54.65 18.20 0.23 74.30 7.28                   S-D 0.55 0.68 4.29 4.60 5.34 2.73 4.23 0.13 5.52 1.53                        COMPOUND #5 50 mg/kg                                                           MEAN                                                                               7.88  3.00  58.10 31.05 35.73                                                                              58.6                                                                               15.38   0.13    77.78                                                                               6.73                    S-D 0.82 0.41 3.11 3.20 3.38 3.77 1.44 0.05 2.63 1.92                          P 0.057 0.043 0.028 0.023 0.033 0.101 0.191 0.046 0.198 0.357                COMPOUND #5 100 mg/kg                                                          MEAN                                                                               7.58  4.68  51.43 36.30 37.50                                                                              56.10                                                                              21.58   0.50    67.65                                                                               10.28                   S-D 1.49 2.32 13.87 10.49 10.52 15.70 7.72 0.54 13.39 5.22                     P 0.305 0.155 0.453 0.358 0.269 0.438 0.220 0.144 0.179 0.129                COMPOUND #5 200 mg/kg                                                          MEAN                                                                               6.83  2.90  58.03 32.30 35.80                                                                              65.63                                                                              15.40   0.13    79.30                                                                               5.20                    S-D 0.42 0.14 4.13 4.37 2.86 5.36 4.67 0.05 5.25 0.91                          P 0.326 0.052 0.036 0.060 0.073 0.029 0.230 0.126 0.160 0.071                __________________________________________________________________________

                                      TABLE 7A                                     __________________________________________________________________________     Immunophenotyping of cell subset CD45-CD4-CD8 of COMPOUND #5 in the            spleen                                                                                                  CD4 - CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                             CD45 +                                                                             CD45 +                                                                             CD45 -                                                                             CD45 -                                                                          CD4 + CD8-                                                                    CD4 + CD8+ CD4                                                                 - CD8- CD8+                                                                    CD4- CD4+ CD4-                                                                 CD4+ CD8- CD8+                                                                 CD8- CD8+           __________________________________________________________________________     CTL                                                                            MEAN                                                                               37.38  0.50   56.95  5.23  51.70                                                                              30.05                                                                              9.55                                                                               8.65                                                                               78.45                                                                              5.33                                                                               16.08                                                                              0.13                  S-D 1.42 0.14 1.79 1.08 1.85 1.56 2.66 0.57 2.24 1.03 2.74 0.05              COMPOUND #5 50 mg/kg                                                           MEAN                                                                               36.53  0.63   57.03  5.85  49.85                                                                              29.23                                                                              12.05                                                                              8.83                                                                               75.15                                                                              6.03                                                                               18.65                                                                              0.18                  S-D 5.17 0.25 5.79 0.93 3.12 4.10 6.62 0.93 5.40 0.78 5.95 0.05                                                                          P 0.358 0.171                                                                 0.488 0.151                                                                    0.203 0.330                                                                    0.210 0.380                                                                    0.100 0.108                                                                    0.185 0.091         COMPOUND #5 100 mg/kg                                                          MEAN                                                                               31.78  0.53   63.03  4.68  50.20                                                                              26.40                                                                              16.60                                                                              6.83                                                                               73.48                                                                              4.80                                                                               21.68                                                                              0.10                  S-D 2.71 0.05 3.53 0.94 5.73 2.17 8.27 0.94 7.13 0.92 7.95 0.08                                                                          P 0.015 0.359                                                                 0.015 0.130                                                                    0.303 0.023                                                                    0.086 0.037                                                                    0.131 0.130                                                                    0.120 0.359         COMPOUND #5 200 mg/kg                                                          MEAN                                                                               36.13  0.55   58.40  4.95  52.45                                                                              29.00                                                                              9.98                                                                               8.63                                                                               78.23                                                                              5.08                                                                               16.60                                                                              0.13                  S-D 4.71 0.13 5.32 0.77 1.87 4.39 3.11 0.86 2.47 0.78 2.91 0.05                                                                          P 0.346 0.319                                                                 0.332 0.365                                                                    0.314 0.360                                                                    0.426 0.484                                                                    0.453 0.377                                                                    0.411 0.500         __________________________________________________________________________

                                      TABLE 7B                                     __________________________________________________________________________     Immunophenotyping of cell subset NK-CD8, T.B and CD4-CD11 of COMPOUND #5       in the spleen                                                                                                                      CD4 -                                                                               CD4 -                   NK + CD3- NK + CD3+ NK - CD3- NK - CD3+ TCR(T) Ly5(B) CD4 + CD11b- CD4                                                                + CD11b+ CD11b-                                                                CD11b+                __________________________________________________________________________     CTL                                                                              MEAN 3.43 1.73 54.33 40.55 54.18 39.25 17.65 0.28 78.15 3.93                   S-D 0.53 0.22 3.27 3.86 4.81 5.06 2.78 0.05 2.60 0.64                        COMPOUND #5 50 mg/kg                                                           MEAN                                                                               3.83  2.18  54.38 39.60 51.58                                                                              41.20                                                                              14.85   0.38    81.15                                                                               3.60                    S-D 0.33 0.34 5.08 5.22 5.63 3.11 2.15 0.05 2.43 0.47                          P 0.180 0.0007 0.489 0.273 0.249 0.283 0.159 0.000 0.155 0.176               COMPOUND #5 100 mg/kg                                                          MEAN                                                                               4.10  2.25  65.05 28.65 40.33                                                                              48.28                                                                              14.65   0.33    81.53                                                                               3.53                    S-D 0.80 0.31 4.04 3.35 2.05 2.88 0.87 0.10 1.51 0.81                          P 0.079 0.063 0.013 0.012 0.007 0.038 0.088 0.248 0.092 0.222                COMPOUND #5 200 mg/kg                                                          MEAN                                                                               4.35  2.35  61.10 32.15 47.23                                                                              46.50                                                                              13.10   0.33    78.38                                                                               3.20                    S-D 0.56 0.54 5.04 4.23 5.78 5.15 2.51 0.05 9.55 0.39                          P 0.050 0.076 0.045 0.032 0.041 0.012 0.076 0.196 0.481 0.046                __________________________________________________________________________

2. Mitogenic proliferation of T and B cells (spleen)

Peptide #1 (SEQ. ID NO:1) is also shown to activate T and B cells in spleen in vivo/ex vivo (see FIGS. 1A, 1B, 1C and 1D).

3. NK assay

Important in immunosurveillance, NK cells are capable of lysing tumors, virus-infected or embryo-derived cells in vitro spontaneously without prior sensitization. Immunomodulators are evaluated using murine splenocytes and human peripheral blood mononuclear leukocytes.

Spleen cells were used to test for NK activity at a effector:target ratio of 100:1.

                  TABLE 8                                                          ______________________________________                                         Functional in vivo/ex vivo NK cell assay of Peptide #1 (SEQ.                     ID NO:1) in mice.                                                                                         % response                                                                             % response                                  Group Average S.D. of IL-2 of CTL                                            ______________________________________                                         Control   2.42    0.69     42.98   100.00                                        IL-2 5.22 -- 100.00 233.04                                                     50 mg/kg 2.52 0.11 48.34 112.48                                                100 mg/kg 3.63 0.94 69.48 161.66                                               200 mg/kg 2.77 0.74 53.13 123.63                                               300 mg/kg 3.05 0.11 58.49 136.11                                             ______________________________________                                    

EXAMPLE 4 Toxicity

peptide #1 (SEQ. ID NO:1) appears to be non-toxic. No apparent toxicity was observed in vivo when animals were treated with 50, 100, 200, and 300 mg/kg of the peptide.

No toxic effect was recorded for peptide #5 (SEQ. ID NO:1) up to 200 mg/kg. Gross pathological observations demonstrated no sign of irritation.

Example 5 Stability of peptide #5 (SEQ. ID NO:1) in Rat Whole Blood at 37° C. METHOD

1. ANALYTICAL PROCEDURE

Column: YMC phenyl, 3 mm, 120 A°, 4.6×150 mm

Flow Rate: 0.5 ml/minute

Activation: 205 nm

Eluent: A=CH₃ CN B=Ammonium Acetate 0.01 M pH 6

Program: 0-5 minutes 5% A/B 5-25 minutes 5%→15% A/B Curve 6 25-30 minutes 15%→50% A/B Curve 6

Attenuation: 0.3 AU

Injection: 30 ml

2. BLOOD INCUBATION

Stock solution of the peptide is prepared at a concentration of 1.lmg/ml and dissolved in 10% CH₃ CN/H₂ O.

Rat Whole Blood (EDTA) is spiked with the peptide to give a concentration of 55 mg/ml.

Same concentration of 10% CH₃ CN/H₂ O is also spiked to Rat Whole Blood (EDTA) as a control blank.

Aliquotes of 500ml are taken (in duplicate) of both spiked and control blanks at the following times:

FOR SPIKED BLOOD: 0', 5', 10', 15', 30', 45', 60', 75', 90'.

FOR BLANK BLOOD: 0', 90'.

Each aliquoted samples are centrifuged at 4° C., 4400 rpm for 10 minutes.

3. Extraction Procedure: Liquid--Liquid Extraction

Protein Precipitation

For each plasma sample, 100 μl of plasma are transferred to an eppendorf vial and 200 μl of acetonitrile added to it. The mixture is vortexed for 30 seconds and centrifuged at 4° C., 5 minutes 14000 rpm (Centra MP4R centrifuge, rotor # IEC 851). 200 μl of supernatant are transferred to another vial and evaporated to dryness with the Speed vac Plus (Savant SC-110A). The dried sample is reconstituted with 100 ml of Mobile phase and an aliquote of this is injected to HPLC(high pressure liquid chromatography) for analysis.

Results

The results of the HPLC analysis are presenred on Table 9.

1. peptide #5 (SEQ. ID NO:1) has a preliminary Extraction Efficiency of 69%±7%.

2. peptide #5(SEQ. ID NO:1) has a half life in Rat Whole Blood>90 minutes.

                  TABLE 9                                                          ______________________________________                                                Time                                                                      (Min.) Area                                                                  ______________________________________                                                0             2471958                                                     5 2362251.2                                                                    10 2386700                                                                     15 2965610                                                                     30 2038700.5                                                                   45 2186985.5                                                                   60 2194185.5                                                                   75 2559208.5                                                                   90 2263081.5                                                                 ______________________________________                                    

Example 6 Stability of Peptide #1 (SEQ. ID. NO:1) and Peptide #5(SEQ. ID NO:1) in Human Whole Blood at 37° C.

The procedures are the same as example 5 with the exceptions that human whole blood was substituted for rat whole blood, and that in Step 2, (blood incubation) aliquotes were taken at the following times:

FOR SPIKED BLOOD: 0', 15', 30', 60', 90', 120', 150'180' and 210'.

FOR BLANK BLOOD: 0', 60', 180', 210.

RESULTS

Thr results of the HPLC analysis are presented on Table 10.

1. peptide #1 (SEQ. ID NO:1) has a half life in Human Whole Blood (37° C.)⁻ 20 min.

2. peptide #5 (SEQ. ID NO:1) has a half life in Human Whole Blood (37° C.)>210 min.

                  TABLE 10                                                         ______________________________________                                                    PEPTIDE #1  PEPTIDE #5                                                Time (SEQ. ID NO:1) (SEQ. ID NO:1)                                           (Min.)     AREA                                                                ______________________________________                                         0          887657      2857082.5                                                 15 354842 3066465                                                              30 127725 3017509.5                                                            45 0 --                                                                        60 --  3135696.5                                                               90 -- 3005211                                                                  120 -- 3476665.5                                                               150 -- 3063052                                                                 180 -- 3108157                                                                 210 -- 3181899                                                               ______________________________________                                    

    __________________________________________________________________________     #             SEQUENCE LISTING                                                    - -  - - (1) GENERAL INFORMATION:                                              - -    (iii) NUMBER OF SEQUENCES: 6                                            - -  - - (2) INFORMATION FOR SEQ ID NO:1:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                - - Lys Asn Pro Tyr                                                           1                                                                               - -  - - (2) INFORMATION FOR SEQ ID NO:2:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                - - Lys Asn Gly Tyr                                                           1                                                                               - -  - - (2) INFORMATION FOR SEQ ID NO:3:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                - - Lys Asn Pro Phe                                                           1                                                                               - -  - - (2) INFORMATION FOR SEQ ID NO:4:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                - - Lys Gln Pro Tyr                                                           1                                                                               - -  - - (2) INFORMATION FOR SEQ ID NO:5:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                - - Gly Lys Asn Pro Tyr                                                       1               5                                                               - -  - - (2) INFORMATION FOR SEQ ID NO:6:                                      - -      (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 4 amino - #acids                                                   (B) TYPE: amino acid                                                           (C) STRANDEDNESS:                                                              (D) TOPOLOGY: linear                                                  - -     (ii) MOLECULE TYPE: peptide                                            - -      (v) FRAGMENT TYPE: linear                                             - -     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:                                - - Gly Asn Pro Tyr                                                         __________________________________________________________________________ 

We claim:
 1. A peptide of the formula (I):

    X-R.sub.1 -R.sub.2 -R.sub.3 -R.sub.4 -Y

wherein; X is selected from the group consisting of H or acetyl; R₁ is selected from the group consisting of D or L-lysine; arginine; ornithine and histidine; R₂ is selected from the group consisting of D or L-asparagine; alanine; proline; glutamine; serine; threonine; and valine; R₃ is selected from the group consisting of D or L-proline; alanine; asparagine; glutamine; serine; threonine; valine; and glycine; R₄ is selected from the group consisting of D or L-tyrosine; cysteine; serine; threonine; phenylalanine; tryptophan; and histidine; and Y is selected from the group consisting of OH, NH₂, and OC₁₋₆ alkyl.
 2. The peptide according to claim 1 wherein X is hydrogen.
 3. The peptide according to claim 1 wherein R₁ is D-lysine.
 4. The peptide according to claim 1 wherein R₂ asparagine.
 5. The peptide according to claim 1 wherein R₂ is glutamine.
 6. The peptide according to claim 1 wherein R₃ is D-Proline.
 7. The peptide according to claim 1 wherein R₄ is tyrosine.
 8. The peptide according to claim 1 wherein R₄ is phenylalanine.
 9. The peptide according to claim 1 wherein Y is OH.
 10. A pharmaceutical composition containing a peptide according to claim 1, wherein said peptide is present in admixture with a pharmaceutically acceptable carrier.
 11. The pharmaceutical composition according to claim 10 wherein peptide is present in admixture with another therapeutically active agent.
 12. A method for the treatment of immune deficiencies comprising the step of administering to a mammal, including human, a pharmaceutically acceptable amount of a peptide according to claim
 1. 13. A method for stimulating B-cells comprising the step of administering to a mammal, including human, a pharmaceutically acceptable amount of a peptide according to claim
 1. 14. The method according to claim 13, wherein said peptide is administered in an amount ranging from about 0.1 to about 750 mg/kg.
 15. A method of manufacturing a medicament for the treatment of immune deficiencies, using a peptide according to claim 1, in an amount effective to produce an immune response.
 16. A peptide selected from the group consisting of:peptide #1 H-Lys-Asn-Pro-Tyr-OH (SEQ ID NO:1); peptide #2 H-Lys-Asn-Gly-Tyr-OH (SEQ ID NO:2); peptide #3 H-Lys-Asn-(D-Pro)-Tyr-OH (SEQ ID NO:1); peptide #4H-Lys-Asn-Pro-Phe-OH (SEQ ID NO:3); peptide #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ ID NO:1); peptide #6 H-Lys-Gln-Pro-Tyr-OH (SEQ ID NO:4); peptide #8 H-Gly-Asn-Pro-Tyr-OH (SEQ ID NO:6); peptide #9 H-(D-Lys)-Asn-Gly-Tyr-OH (SEQ ID NO:2); peptide #10 H-(D-Lys)-Gln-Pro-Tyr-OH (SEQ ID NO:4); and peptide #11 H-(D-Lys)-Asn-Pro-Phe-OH (SEQ ID NO:3).
 17. The peptide according to claim 16 selected from the group consisting of:peptide #1 (SEQ. ID NO:1) H-Lys-Asn-Pro-Tyr-OH; (SEQ. ID NO:1) and peptide #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ. ID NO:1).
 18. The peptide according to claim 17 of the formula:peptide #1 H-Lys-Asn-Pro-Tyr-OR (SEQ. ID NO:1).
 19. The peptide according to claim 17 of the formula:peptide #5 H-(D-Lys)-Asn-Pro-Tyr-OH (SEQ. ID NO:1). 